Coding

Part:BBa_K2637005:Design

Designed by: Haoqian Zhao   Group: iGEM18_Tianjin   (2018-10-05)


CikA Protein of Circadian Rhythm


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.

Source

CikA is from the the cyanobacterium Synechococcus elongatus PCC7942 and naturally controls the phosphorylation of RpaA. In vivo, CikA will bind to the KaiBC complex during the subjective night and dephosphorylate RpaA via its phosphatase activities.

References